http://blog.ginkgobioworks.com/2009/01/31/ligations-done-right/ By Ginkgo BioWorks Fri, 27 Jan 2012 13:52:55 +0000 hourly 1 http://wordpress.org/?v=3.3.1 http://blog.ginkgobioworks.com/2009/01/31/ligations-done-right/comment-page-1/#comment-6135 Dan Sullivan Fri, 13 Mar 2009 00:31:03 +0000 http://blog.ginkgobioworks.com/?p=91#comment-6135 The NEB ligase & ligase buffer are great but before you use the thawed buffer, be sure there is no undissolved precipitate in the tube. If you do see little white bits in the tube, warm it up and keep inverting it until its all dissolved. The NEB ligase & ligase buffer are great but before you use the thawed buffer, be sure there is no undissolved precipitate in the tube. If you do see little white bits in the tube, warm it up and keep inverting it until its all dissolved.

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